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产品描述:人乳糖凝集素3结合蛋白(LGALS3BP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的LGALS3BP含量。LGALS3BP 是当前受关注的靶点。它在多种生理与病理过程中发挥作用。研究聚焦其在肿瘤等疾病中的机制,如参与肿瘤细胞黏附、转移等。对它的深入研究,有望为相关疾病的诊断、治疗提供新思路和靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL,支持科研人员探究LGALS3BP在肿瘤生物学机制、炎症相关疾病模型或病原体宿主相互作用中的表达规律;可用于药物干预实验中生物标志物的动态监测;为生命科学基础研究提供可靠的定量分析工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
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缩写:LGALS3BP
Uniprot No.:Q08380
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:1.56 ng/mL-100 ng/mL
灵敏度:0.39 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cell Biology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human LGALS3BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:20 | Average % | 91 | ||||
Range % | 84-98 | |||||
1:40 | Average % | 90 | ||||
Range % | 85-95 | |||||
1:60 | Average % | 97 | ||||
Range % | 92-102 | |||||
1:160 | Average % | 102 | ||||
Range % | 96-108 |
回收率:
The recovery of human LGALS3BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 100 | 95-106 | ||||
EDTA plasma (n=4) | 90 | 85-95 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected 1002.6202.6452.6332.541 502.2652.3662.3162.224 251.6281.6731.6511.559 12.50.9210.8550.8880.796 6.250.5710.5890.5800.488 3.120.3330.3550.3440.252 1.560.1900.1880.1890.097 00.0930.0900.092 |
货期:3-5 working days
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