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产品描述：牛生长分化因子8(MSTN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MSTN含量。MSTN 即肌肉生长抑制素，是调控肌肉发育的关键负性因子。其背景在于它的正常表达能维持肌肉量平衡。研究机制方面，它可抑制肌肉干细胞增殖分化，减少成肌细胞生成及肌纤维生长。对其深入研究有望为肌肉萎缩等疾病治疗带来突破。试剂盒检测范围为6.25 ng/mL-400 ng/mL，广泛应用于畜牧科学领域，包括动物肌肉生长调控研究、遗传育种效果评估、营养干预实验以及肌肉相关疾病模型的分子机制探索，为科研人员提供可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MSTN ELISA kit; GDF8 ELISA kit; MHGrowth/differentiation factor 8 ELISA kit; GDF-8 ELISA kit; Myostatin ELISA kit
缩写：MSTN
Uniprot No.：O18836
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine MSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 88 | |||||
| Range % | 82-93 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 90-101 | ||||||
| 1:4 | Average % | 98 | |||||
| Range % | 88-103 | ||||||
| 1:8 | Average % | 104 | |||||
| Range % | 97-109 | ||||||
回收率：
| The recovery of bovine MSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 99 | 91-105 | |||||
| EDTA plasma (n=4) | 89 | 81-94 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected4001.9541.9021.9281.8052001.5341.4871.5111.3881001.0041.0561.0300.907500.6520.6720.6620.539250.4160.4050.4110.28812.50.2850.2730.2790.1566.250.2170.2120.2150.09200.1250.1210.123 | |||||||
货期：3-5 working days
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