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产品描述：鱼金属硫蛋白(MT)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的MT含量。试剂盒检测范围为25 pg/mL-400 pg/mL，适用于水产养殖研究、水生态系统重金属污染监测、鱼类生理应激机制分析等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MT
种属：Fish
样本类型：serum, plasma
检测范围：25 pg/mL-400 pg/mL
灵敏度：12.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish MT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:200 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 94-103 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of fish MT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-102 | ||||
| EDTA plasma (n=4) | 95 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 4000.1030.1000.102 2000.2470.2280.238 1000.4010.3870.394 500.6460.5980.622 250.9830.9660.975 01.5721.5291.551 | ||||||
货期：3-5 working days
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