首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠胃动素(MTL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MTL含量。大鼠胃动素（MTL）是一种由内分泌Mo细胞分泌的22个氨基酸组成的活性肽，主要在消化间期周期性释放，促进胃肠运动和胃蛋白酶分泌。其作用机制涉及与胃肠平滑肌上的胃动素受体结合，增强平滑肌收缩，加速胃排空，并调节消化间期胃肠活动，如移行性复合运动（MMC）。MTL的研究对于理解胃肠动力和消化功能具有重要意义。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于胃肠动力障碍动物模型研究、药物干预后胃动素分泌评估，以及消化道组织样本中MTL表达的动态监测，为胃肠生理学、病理学机制研究及药效学评价提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：MTL
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MTL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-96 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 96-106 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-102 | |||||
回收率：
| The recovery of rat MTL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 98-108 | ||||
| EDTA plasma (n=4) | 96 | 92-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.7252.9052.8152.61410002.2222.1022.1621.9615001.5271.5071.5171.3162500.9200.9300.9250.7241250.5770.5890.5830.38262.50.4320.4230.4280.22731.250.3020.2960.2990.09800.1970.2040.201 | ||||||
货期：3-5 working days
×
