首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：猪粘蛋白/粘液素2(MUC2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MUC2含量。MUC2是一种分泌型粘蛋白，主要在小肠和结肠的杯状细胞中表达。它在维持肠道稳态中发挥重要作用，其破坏与多种疾病和癌症有关。MUC2的研究涉及其在肠道黏膜屏障损伤中的作用，以及其基因表达调控机制。试剂盒检测范围为7.8 ng/mL-500 ng/mL，该产品适用于猪肠道黏膜屏障功能研究、消化道疾病模型中的黏液分泌机制探索，以及营养干预对肠道健康影响的评估等科研领域，尤其适合通过多类型样本（如肠道组织匀浆与体液样本）对比分析MUC2的动态变化，为猪的肠道生理病理研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MUC2
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig mucin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 87-94 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 85-95 | |||||
回收率：
| The recovery of pig mucin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-96 | ||||
| EDTA plasma (n=4) | 98 | 95-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected5001.9762.1212.0491.8852501.3031.2101.2571.0931250.7740.7840.7790.61562.50.4280.4250.4270.26331.250.2960.2740.2850.12115.620.2350.2500.2430.0797.80.2080.2070.2080.04400.1580.1690.164 | ||||||
货期：3-5 working days
×
