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产品描述：小鼠粘蛋白/粘液素5AC(MUC5AC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MUC5AC含量。MUC5AC是一个重要靶点。它是一种黏蛋白，与呼吸系统等疾病密切相关，如哮喘、慢性阻塞性肺疾病等。研究机制主要聚焦于其在疾病发生发展中参与调节黏液分泌、影响炎症反应等，对它的深入研究有望为相关疾病治疗提供新策略。试剂盒检测范围为4.688 ng/mL-300 ng/mL，本试剂盒适用于体外实验研究，如评估病理状态下粘液分泌调节机制、筛选影响粘蛋白表达的化合物或药物，以及探究感染性疾病中宿主防御反应等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MUC5AC
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：4.688 ng/mL-300 ng/mL
灵敏度：1.172 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MUC5AC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 91-102 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 93-103 | |||||
回收率：
| The recovery of mouse MUC5AC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 91-104 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected3002.2752.1822.2292.0571501.4631.4341.4491.277750.8550.8180.8370.66537.50.5240.5070.5160.34418.750.3510.3290.3400.1689.3750.2810.2620.2720.1004.6880.2350.2170.2260.05400.1740.1690.172 | ||||||
货期：3-5 working days
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