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Human myosin light chain kinase,MLCK ELISA Kit

  • 中文名： 人肌球蛋白轻链激酶(MLCK)ELISA Kit
货号: IPD-E09048h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人肌球蛋白轻链激酶(MLCK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MYLK含量。MYLK（肌球蛋白轻链激酶）是一种钙/钙调蛋白依赖性酶，主要参与平滑肌的紧张性收缩和细胞对钙离子的反应。其研究机制涉及磷酸化肌球蛋白调节轻链，促进肌动蛋白与肌球蛋白相互作用，从而产生收缩活性。试剂盒检测范围为0.78 ng/mL-50 ng/mL，科研人员可借助该产品探索MLCK在平滑肌功能异常相关疾病（如高血压、肠动力障碍）中的分子机制，或研究肿瘤微环境中细胞迁移的调控路径，为细胞信号转导、病理模型构建及药物靶点筛选提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    deglutamylated form ELISA Kit; DKFZp686I10125 ELISA Kit; EC 2.7.11.18 ELISA Kit; FLJ12216 ELISA Kit; Kinase related protein ELISA Kit; Kinase-related protein ELISA Kit; KRP ELISA Kit; MLCK ELISA Kit; MLCK1 ELISA Kit; MLCK108 ELISA Kit; MLCK210 ELISA Kit; MSTP083 ELISA Kit; MYLK ELISA Kit; MYLK_HUMAN ELISA Kit; MYLK1 ELISA Kit; Myosin light chain kinase ELISA Kit; Myosin light polypeptide kinase ELISA Kit; OTTHUMP00000180642 ELISA Kit; OTTHUMP00000180643 ELISA Kit; smMLCK ELISA Kit; smooth muscle ELISA Kit; Smooth muscle myosin light chain kinase ELISA Kit; Telokin ELISA Kit
  • 缩写：
    MYLK
  • Uniprot No.：
    Q15746
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human MLCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    82


    Range %

    80-85


    1:2

    Average %

    97


    Range %

    94-100


    1:4

    Average %

    87


    Range %

    82-92


    1:8

    Average %

    97


    Range %

    91-103


  • 回收率：

    The recovery of human MLCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    89

    86-91


    EDTA plasma (n=4)

    104

    100-108


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    50

    2.420

    2.468

    2.444

    2.302


    25

    1.957

    1.969

    1.963

    1.821


    12.5

    1.298

    1.279

    1.289

    1.147


    6.25

    0.764

    0.728

    0.746

    0.604


    3.12

    0.368

    0.352

    0.360

    0.218


    1.56

    0.288

    0.298

    0.293

    0.151


    0.78

    0.202

    0.204

    0.203

    0.061


    0

    0.140

    0.144

    0.142




  • 货期：
    3-5 working days

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