首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NAG含量。试剂盒检测范围为15.6 μIU/mL-1000 μIU/mL，适用于基础医学研究中NAG相关代谢通路探索、药物肾毒性评估、肾脏疾病动物模型构建等科研领域，特别适合用于分析肾小管损伤模型或肝脏代谢研究中NAG的动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hexb ELISA Kit; Beta-hexosaminidase subunit beta ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit beta ELISA Kit; Hexosaminidase subunit B ELISA Kit; N-acetyl-beta-glucosaminidase subunit beta ELISA Kit
缩写：NAG
Uniprot No.：P20060
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 μIU/mL-1000 μIU/mL
灵敏度：3.9 μIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:10 | Average % | 93 | ||||
| Range % | 85-99 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 92-98 | |||||
| 1:40 | Average % | 90 | ||||
| Range % | 87-100 | |||||
| 1:80 | Average % | 88 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of mouse NAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 86-101 | ||||
| EDTA plasma (n=4) | 95 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μIU/mlOD1OD2AverageCorrected 10002.5552.5292.5422.398 5002.0842.1052.0951.951 2501.4421.4671.4551.311 1250.9460.9550.9510.807 62.50.4130.4080.4110.267 31.20.3210.3260.3240.180 15.60.2440.2390.2420.098 00.1420.1450.144 | ||||||
货期：3-5 working days
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