首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠中性粒细胞弹性蛋白酶(NE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的NE含量。靶点研究是药物开发的关键，旨在识别疾病过程中的关键分子，为药物设计提供方向。研究背景包括疾病机制、靶点数据库和筛选验证方法。机制研究则涉及靶点与药物相互作用，以及其在疾病治疗中的作用。试剂盒检测范围为12.5 ng/mL-800 ng/mL，该产品适用于基础科研领域，如炎症机制研究、药物干预效果评估或疾病模型（如呼吸系统疾病、关节炎等）中NE动态变化的监测，为探究NE在病理生理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bone marrow serine protease ELISA Kit; ELA2 ELISA Kit; ELANE ELISA Kit; Elastase 2 ELISA Kit; Elastase 2 neutrophil ELISA Kit; Elastase neutrophil expressed ELISA Kit; Elastase-2 ELISA Kit; ELNE_HUMAN ELISA Kit; GE ELISA Kit; Granulocyte derived elastase ELISA Kit; HLE ELISA Kit; HNE ELISA Kit; Human leukocyte elastase ELISA Kit; Leukocyte elastase ELISA Kit; Medullasin ELISA Kit; NE ELISA Kit; Neutrophil elastase ELISA Kit; PMN E ELISA Kit; PMN elastase ELISA Kit; Polymorphonuclear elastase ELISA Kit; SCN1 ELISA Kit
缩写：NE
Uniprot No.：P08246
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：12.5 ng/mL-800 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 83-93 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 83-95 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-97 | |||||
回收率：
| The recovery of rat NE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-102 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected8002.8052.7672.7862.5844002.4622.3742.4182.2162002.1312.0892.1101.9081001.6691.5781.6241.422501.2131.1561.1850.983250.8570.7930.8250.62312.50.4460.4240.4350.23300.2040.1990.202 | ||||||
货期：3-5 working days
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