首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠神经丝蛋白轻链多肽(NEFL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NEFL含量。NEFL 即神经丝轻链蛋白，是神经元细胞骨架的组成成分。其背景上，它在神经系统疾病中具有重要意义。研究机制方面，血液和脑脊液中的 NEFL 水平变化能反映神经元损伤情况，可作为多种神经退行性疾病早期诊断和病情监测的生物标志物。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于神经科学领域基础研究，包括中枢神经系统疾病动物模型构建、神经再生机制探索、神经毒性物质评估等场景，尤其可为脑损伤模型、脊髓病变研究及神经保护药物开发提供可靠的定量检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nefl ELISA Kit; Nf68 ELISA Kit; Nfl ELISA Kit; Neurofilament light polypeptide ELISA Kit; NF-L ELISA Kit; 68 kDa neurofilament protein ELISA Kit; Neurofilament triplet L protein ELISA Kit
缩写：NEFL
Uniprot No.：P19527
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NEFL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 85-98 | |||||
| 1:2 | Average % | 107 | ||||
| Range % | 97-111 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 83-94 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of rat NEFL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 82-94 | ||||
| EDTA plasma (n=4) | 99 | 91-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.5162.4112.4642.2872502.0521.9872.0201.8431251.2971.2821.2901.11362.50.7250.7840.7550.57831.20.4420.4370.4400.26315.60.3380.3300.3340.1577.80.2590.2490.2540.07700.1780.1760.177 | ||||||
货期：3-5 working days
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