首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠核因子E2相关因子2(Nrf2)酶联免疫试剂盒，双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的NFE2L2含量。NFE2L2，也称为NRF2，是一种转录因子，属于碱性亮氨酸拉链（bZIP）蛋白家族。它通过结合抗氧化反应元件（ARE）来调控基因表达，参与氧化应激反应和细胞保护。NFE2L2的异常表达与多种疾病，包括癌症和神经退行性疾病有关。其研究机制主要涉及NFE2L2的激活、调控及其在细胞抗氧化和铁死亡中的作用。试剂盒检测范围为0.312 ng/mL-20 ng/mL，可用于研究氧化应激相关信号通路、评估药物或化合物对Nrf2表达的影响，亦可用于细胞模型中Nrf2动态变化的监测，为神经退行性疾病、炎症反应及肿瘤微环境等领域的分子机制探索提供技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nfe2l2 ELISA Kit; Nrf2 ELISA Kit; Nuclear factor erythroid 2-related factor 2 ELISA Kit; NF-E2-related factor 2 ELISA Kit; NFE2-related factor 2 ELISA Kit; Nuclear factor ELISA Kit; erythroid derived 2 ELISA Kit; like 2 ELISA Kit
缩写：NFE2L2
Uniprot No.：Q60795
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Nrf2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 83-94 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 96-107 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 93-104 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-99 | |||||
回收率：
| The recovery of mouse Nrf2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 82-95 | ||||
| EDTA plasma (n=4) | 95 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.6482.8802.7642.627101.9661.9161.9411.80451.3571.2911.3241.1872.50.9630.9450.9540.8171.250.5140.5470.5310.3940.6250.3580.3450.3520.2150.3120.2330.2390.2360.09900.1380.1350.137 | ||||||
货期：3-5 working days
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