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产品描述：绵羊中性粒细胞明胶酶相关脂质运载蛋白(NGAL)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NGAL含量。NGAL即中性粒细胞明胶酶相关脂质运载蛋白，是一种小分子分泌型蛋白。作为新兴靶点，其与细胞增殖、凋亡、迁移等相关。研究机制主要围绕它在炎症、肿瘤等疾病中的表达变化，以及与其他分子相互作用对疾病进程的影响。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，在绵羊疾病模型研究中具有重要价值；适用于绵羊炎症机制研究、肾脏疾病动物模型建立、药物干预效果评价等科研领域；为实验室开展NGAL相关病理生理研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：NGAL
种属：Ovis aries (Sheep)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep NGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 86-95 | |||||
| 1:400 | Average % | 103 | ||||
| Range % | 99-105 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 88-98 | |||||
| 1:1600 | Average % | 99 | ||||
| Range % | 94-105 | |||||
回收率：
| The recovery of sheep NGAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-97 | ||||
| EDTA plasma (n=4) | 95 | 88-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average10000.1150.1180.1175000.2270.2310.2292500.4830.5040.4941250.9561.0390.99862.51.4251.4381.43231.252.3542.3662.36015.62.6522.6712.66202.9272.9452.936 | ||||||
货期：3-5 working days
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