Mouse Nerve growth factor,NGF ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E04684m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠神经生长因子(NGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的NGF含量。NGF即神经生长因子，是神经营养因子家族一员，在神经元生长、发育、存活及维持正常功能中起关键作用。研究机制包括与特异性受体结合启动细胞内信号传导通路，调节基因表达等，对神经系统疾病治疗有潜在意义。试剂盒检测范围为62.5 pg/ml-4000 pg/ml，适用于神经科学领域的多种研究场景，包括神经损伤动物模型评估、体外神经元培养体系中神经营养因子动态监测、神经退行性疾病机制探究以及药物干预效果分析等。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Ngf ELISA Kit; Ngfb ELISA Kit; Beta-nerve growth factor ELISA Kit; Beta-NGF ELISA Kit
缩写：
NGF
Uniprot No.：
P01139
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates.
检测范围：
62.5 pg/ml-4000 pg/ml
灵敏度：
15.6 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Neuroscience
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-102
1:2	Average %	91
	Range %	85-98
1:4	Average %	90
	Range %	88-93
1:8	Average %	100
	Range %	96-104

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
4000	2.802	2.752	2.777	2.610
2000	2.439	2.376	2.408	2.241
1000	1.786	1.662	1.724	1.557
500	1.018	1.009	1.014	0.847
250	0.531	0.528	0.530	0.363
125	0.388	0.359	0.374	0.207
62.5	0.278	0.289	0.284	0.117
0	0.168	0.165	0.167