首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠NACHT,LRR和PYD结构域蛋白3(NLRP3)酶联免疫试剂盒，为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NLRP3含量。NLRP3是重要靶点。它是细胞内模式识别受体，参与天然免疫反应。在多种疾病中异常激活，如炎症性疾病、神经退行性疾病等。其研究机制主要围绕激活途径及调控因素等展开，旨在找到调控它的方法，为相关疾病治疗提供思路。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于体外实验中对炎症相关信号通路的研究，如巨噬细胞活化分析、模式识别受体功能探索、炎症因子级联反应机制解析等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nlrp3 ELISA Kit; Cias1 ELISA Kit; Mmig1 ELISA Kit; Nalp3 ELISA Kit; Pypaf1 ELISA Kit; NACHT ELISA Kit; LRR and PYD domains-containing protein 3 ELISA Kit; Cold autoinflammatory syndrome 1 protein homolog ELISA Kit; Cryopyrin ELISA Kit; Mast cell maturation-associated-inducible protein 1 ELISA Kit; PYRIN-containing APAF1-like protein 1 ELISA Kit
缩写：NLRP3
Uniprot No.：Q8R4B8
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NLRP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-96 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-95 | |||||
回收率：
| The recovery of mouse NLRP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-96 | ||||
| EDTA plasma (n=4) | 100 | 96-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 4002.2172.4062.3122.138 2001.2411.3021.2721.098 1000.7370.7620.7500.576 500.4490.4780.4640.290 250.3730.3470.3600.186 12.50.2970.2800.2890.115 6.250.2050.2030.2040.030 00.1700.1780.174 | ||||||
货期：3-5 working days
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