首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠内皮型一氧化氮合成酶(eNOS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NOS3含量。NOS3即内皮型一氧化氮合酶，在血管内皮细胞中表达。其背景在于它催化生成的一氧化氮对血管舒张、抑制血小板聚集等生理过程至关重要。研究机制多围绕其活性调控、基因表达等，探究其在心血管疾病等病理过程中的作用及潜在治疗靶点价值。试剂盒检测范围为0.312 IU/mL-20 IU/mL，适用于实验室研究中对小鼠心血管功能相关模型的分子机制探索、药物干预效果评估或基因表达调控分析，可为动物实验中的组织、体液样本提供高效检测方案，满足基础科研中蛋白质表达定量需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nos3 ELISA Kit; EcnosNitric oxide synthase ELISA Kit; endothelial ELISA Kit; EC 1.14.13.39 ELISA Kit; Constitutive NOS ELISA Kit; cNOS ELISA Kit; EC-NOS ELISA Kit; Endothelial NOS ELISA Kit; eNOS ELISA Kit; NOS type III ELISA Kit; NOSIII ELISA Kit
缩写：NOS3
Uniprot No.：P70313
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 IU/mL-20 IU/mL
灵敏度：0.078 IU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse eNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 82-95 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-103 | |||||
| 1:4 | Average % | 105 | ||||
| Range % | 98-112 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of mouse eNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-104 | ||||
| EDTA plasma (n=4) | 102 | 94-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| IU/mlOD1OD2AverageCorrected 202.9952.8652.9302.728 102.2802.2442.2622.060 51.5281.5461.5371.335 2.50.9260.9400.9330.731 1.250.5980.5780.5880.386 0.6250.4450.4310.4380.236 0.3120.2980.3050.3020.100 00.1990.2040.202 | ||||||
货期：3-5 working days
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