Mouse Nephrin(NPHS1) ELISA kit

货号: IPD-EL015988MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：小鼠人肾病蛋白(NPHS1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NPHS1含量。NPHS1 是一个重要靶点。它是编码足细胞裂孔隔膜蛋白 Nephrin 的基因。研究机制方面，其突变会导致 Nephrin 蛋白异常，破坏裂孔隔膜结构和功能，引发蛋白尿等肾脏疾病。对它的研究有助于深入了解肾脏疾病发病机制和开发新疗法。试剂盒检测范围为250 pg/mL-4000 pg/mL，适用于科研领域对小鼠或人源化疾病模型中NPHS1蛋白表达动态的监测，例如肾脏损伤模型构建、药物干预后肾小球功能评估或遗传性肾病机制研究。兼容多种样本类型，可满足体外实验中对血清循环蛋白水平检测或组织局部表达分析的多元化需求，为探索NPHS1在肾脏疾病中的调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nphs1 ELISA Kit; Nphn ELISA Kit; Nephrin ELISA Kit; Renal glomerulus-specific cell adhesion receptor ELISA Kit
缩写：NPHS1
Uniprot No.：Q9QZS7
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：250 pg/mL-4000 pg/mL
灵敏度：250 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NPHS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	86-98
1:200	Average %	97
	Range %	92-102
1:400	Average %	85
	Range %	82-88
1:800	Average %	88
	Range %	83-94

回收率：

The recovery of mouse NPHS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	94	90-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/mlOD1OD2Average 40000.1100.1150.113 20000.2870.2980.293 10000.5350.5440.540 5000.8620.8480.855 2501.3661.3751.371 02.5022.5232.513