首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠心钠肽(ANP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的NPPA含量。NPPA 即心钠肽前体 A。它在心血管系统中发挥重要作用，能调节血压、血容量等。研究机制上主要围绕其基因表达调控以及生成的利钠肽生理功能开展，探究其在心脏生理与病理过程中的角色，为心血管疾病防治提供新思路。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本试剂盒适用于小鼠模型中ANP水平变化的动态监测，例如心脏负荷实验、高血压模型评估或心脏功能相关的基础研究。可应用于心血管疾病机制探索、药物干预效果评价等实验场景，为动物模型中心功能相关指标提供定量化数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nppa ELISA Kit; PndNatriuretic peptides A ELISA Kit; Prepronatriodilatin) [Cleaved into: Atrial natriuretic factor ELISA Kit; ANF ELISA Kit; Atrial natriuretic peptide ELISA Kit; ANP); Auriculin-B; Auriculin-A; Atriopeptin-1 ELISA Kit; Atriopeptin I); Atriopeptin-2 ELISA Kit; Atriopeptin II)] ELISA Kit
缩写：NPPA
Uniprot No.：P05125
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 98 | |||||
| Range % | 91-102 | ||||||
| 1:2 | Average % | 107 | |||||
| Range % | 98-111 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 84-96 | ||||||
| 1:8 | Average % | 99 | |||||
| Range % | 93-102 | ||||||
回收率：
| The recovery of mouse ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 84-97 | |||||
| EDTA plasma (n=4) | 92 | 88-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.0452.1052.0751.944101.7431.7941.7691.63851.1671.1991.1831.0522.50.6080.6270.6180.4871.250.3090.3140.3120.1810.6250.2010.2130.2070.0760.3120.1650.1610.1630.03200.1330.1290.131 | |||||||
货期：3-5 working days
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