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产品描述：人神经穿透素-2(NPTX2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NPTX2含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外实验中探索NPTX2在神经退行性疾病模型、神经损伤修复机制或脑组织蛋白表达调控等领域的生物学功能；科研人员可通过定量分析不同病理生理条件下NPTX2的浓度变化，为神经科学基础研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：apexin ELISA Kit; NARP ELISA Kit; neuronal activity-regulated pentaxin ELISA Kit; Neuronal pentraxin 2 ELISA Kit; Neuronal pentraxin II ELISA Kit; Neuronal pentraxin-2 ELISA Kit; NP 2 ELISA Kit; NP-II ELISA Kit; NP2 ELISA Kit; NPII ELISA Kit; NPTX2 ELISA Kit; NPTX2_HUMAN ELISA Kit; pentraxin II, neuronal ELISA Kit
缩写：NPTX2
Uniprot No.：P47972
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NPTX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 91-100 | ||||||
| 1:2 | Average % | 104 | |||||
| Range % | 100-110 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 85-95 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
回收率：
| The recovery of human NPTX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 84 | 80-88 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 102.5202.3852.4532.304 51.5711.4961.5341.385 2.50.9190.9730.9460.797 1.250.5250.5570.5410.392 0.6250.3520.3290.3410.192 0.3120.2680.2540.2610.112 0.1560.1930.2010.1970.048 00.1450.1520.149 | |||||||
货期：3-5 working days
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