首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠神经调节蛋白4(NRG4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NRG4含量。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于科研领域中小鼠模型的基础代谢研究、脂肪组织功能评估或相关疾病机制探索，为体外实验提供高效可靠的工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nrg4 ELISA Kit; Pro-neuregulin-4 ELISA Kit; membrane-bound isoform ELISA Kit; Pro-NRG4) [Cleaved into: Neuregulin-4 ELISA Kit; NRG-4)] ELISA Kit
缩写：NRG4
Uniprot No.：Q9WTX4
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NRG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 92-103 | ||||||
| 1:2 | Average % | 91 | |||||
| Range % | 84-96 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 87-95 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 95-105 | ||||||
回收率：
| The recovery of mouse NRG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 87-93 | |||||
| EDTA plasma (n=4) | 94 | 90-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected2002.5492.7632.6562.5561002.1352.1312.1332.033501.5871.6221.6051.505251.0141.0331.0240.92412.50.7360.7870.7620.6626.250.4680.4360.4520.3523.120.2140.2310.2230.12300.1010.0980.100 | |||||||
货期：3-5 working days
×
