首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠神经秩蛋白(NRTN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NRTN含量。小鼠神经秩蛋白(NRTN)是一种神经营养因子，属于GDNF家族，对神经元生存和功能调节起重要作用。其研究机制涉及通过GFRα/RET受体复合体激活cAMP/PKA途径促进上皮细胞增殖，并参与调节神经生长和稳定性。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于神经科学研究领域，可应用于神经疾病动物模型构建、药物干预效果评估、神经发育调控机制探索等实验场景，为体外定量分析NRTN蛋白表达提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nrtn ELISA Kit; Neurturin ELISA Kit
缩写：NRTN
Uniprot No.：P97463
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NRTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 94-101 | |||||
回收率：
| The recovery of mouse NRTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 97-105 | ||||
| EDTA plasma (n=4) | 89 | 85-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.3102.3682.3392.15710001.5101.6301.5701.3885000.8850.8750.8800.6982500.5000.5310.5160.3341250.3680.3780.3730.19162.50.3080.3010.3050.12331.250.2400.2500.2450.06300.1810.1830.182 | ||||||
货期：3-5 working days
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