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产品描述：猪神经特异性烯醇化酶(NSE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NSE含量。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于脑组织、外周神经等样本的神经功能评估，兼容常规血清/血浆样本（需离心去除沉淀后分装冻存），可满足神经退行性疾病模型构建、神经损伤修复研究以及神经内分泌相关疾病机制探索等科研需求，为动物实验中的病理机制解析和药物干预效果观察提供可靠定量工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：NSE
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-102 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-104 | |||||
回收率：
| The recovery of pig NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 84-102 | ||||
| EDTA plasma (n=4) | 94 | 83-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.7542.6822.7182.614252.0171.9972.0071.90312.51.3301.3621.3461.2426.250.9440.9570.9510.8473.120.6530.6410.6470.5431.560.3780.3690.3740.2700.780.2400.2510.2460.14200.1020.1050.104 | ||||||
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