首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠神经生长因子1(NTN1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NTN1含量。试剂盒检测范围为3.12 pg/mL-200 pg/mL，该产品适用于体外培养细胞分泌研究、神经损伤模型组织分析、以及心血管或肿瘤相关疾病机制探索等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ntn1Netrin-1 ELISA Kit
缩写：NTN1
Uniprot No.：O09118
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NTN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 86-99 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-100 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of mouse NTN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 92-99 | ||||
| EDTA plasma (n=4) | 97 | 91-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 2000.2170.2030.210 1000.3240.3090.317 500.4470.4520.450 250.6350.6710.653 12.50.8600.8730.867 6.251.4111.4481.430 3.121.8481.8341.841 02.7192.7352.727 | ||||||
货期：3-5 working days
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