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产品描述：犬N端前心钠肽(NT-ProANP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的NT-ProANP含量。NT - ProANP是一种重要靶点。它是心房利钠肽原经酶切产生的无活性片段。背景上，其水平变化与心血管疾病密切相关。研究机制方面，检测其浓度可辅助诊断心衰等疾病，能反映心脏功能状态，在心血管疾病诊治中有重要价值。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品可应用于犬类心血管疾病机制研究、心脏功能评估模型构建，以及药物干预或生理病理条件下心脏相关分子变化的动态监测，为动物实验中心脏健康研究提供高效工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：NT-ProANP
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog NT-ProANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 91-105 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 92-106 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of dog NT-ProANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 99 | 95-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected201.8301.8671.8491.645101.5081.5241.5161.31251.1361.1661.1510.9472.50.8290.8120.8210.6171.250.5380.5240.5310.3270.6250.3610.3750.3680.1640.3120.2630.2780.2710.06700.2050.2030.204 | ||||||
货期：3-5 working days
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