首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠N端前脑钠素(NT-proBNP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的NT-proBNP含量。NT-proBNP即N末端B型利钠肽原，是心室肌细胞合成B型利钠肽过程中裂解产生的无活性片段。它在心室容量负荷增加、室壁张力增大时释放增加。临床常通过检测其水平，辅助诊断心力衰竭、评估病情严重程度及预后。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于心脏功能相关研究；在心血管疾病模型构建、药物干预效果评估等科研领域具有重要应用价值；适用于心血管基础研究、转基因动物模型评估、心肌损伤机制探索等科研场景；可为心衰模型、心肌肥厚等实验研究提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：NT-proBNP
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-96 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 91-105 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 85-99 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 83-98 | ||||||
回收率：
| The recovery of mouse NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 87-105 | |||||
| EDTA plasma (n=4) | 95 | 91-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.2352.1992.2172.09051.8841.7491.8171.6902.51.4621.3751.4191.2921.250.9781.0351.0070.8800.6250.5520.5490.5510.4240.3120.2890.2840.2870.1600.1560.2030.2110.2070.08000.1280.1250.127 | |||||||
货期：3-5 working days
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