首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠神经降压素(NTS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NTS含量。试剂盒检测范围为6.25 pg/mL-400 pg/mL，该产品为神经生物学、代谢调控及消化系统疾病研究提供可靠工具，可应用于探究NTS在神经信号传导、能量代谢紊乱或药物干预实验中的作用机制，特别适用于肥胖模型、精神分裂症动物模型及相关分子通路研究中的靶标分子定量分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nts ELISA Kit; Neurotensin/neuromedin N [Cleaved into: Large neuromedin N ELISA Kit; NmN-125); Neuromedin N ELISA Kit; NN ELISA Kit; NmN); Neurotensin ELISA Kit; NT); Tail peptide] ELISA Kit
缩写：NTS
Uniprot No.：Q9D3P9
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NTS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 86 | |||||
| Range % | 83-89 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 94-105 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 85-95 | ||||||
| 1:8 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
回收率：
| The recovery of mouse NTS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 86 | 83-89 | |||||
| EDTA plasma (n=4) | 97 | 94-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 4002.8902.7682.8292.637 2002.3462.2042.2752.083 1001.6261.7021.6641.472 501.0471.1001.0740.882 250.7330.7410.7370.545 12.50.5170.5020.5100.318 6.250.3710.3820.3770.185 00.1910.1930.192 | |||||||
货期：3-5 working days
×
