首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Ⅰ型胶原N末端肽(NTX)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NTX含量。小鼠Ⅰ型胶原N末端肽（NTX）是Ⅰ型胶原蛋白的N端肽段，具有特征性的D-Band结构，对骨生物矿化至关重要。NTX在骨修复材料中发挥重要作用，其稳定的三螺旋结构和自组装能力对形成具有生物矿化能力的胶原纤维至关重要。试剂盒检测范围为3.12 nM BCE-200 nM BCE，常用于评估骨质疏松、骨折修复及骨重塑相关机制的研究；适用于体外实验中对骨代谢疾病模型、药物干预效果或骨骼发育调控的科研分析，可为骨生物学、药理学及再生医学领域提供可靠的数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：cross linked N-telopeptide of type 1 collagen,NTX,_x000D_ cross linked N-telopeptide of type I collagen,NTX
缩写：NTX
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 nM BCE-200 nM BCE
灵敏度：0.78 nM BCE
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NTX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 82-95 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-103 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 96-108 | |||||
回收率：
| The recovery of mouse NTX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-96 | ||||
| EDTA plasma (n=4) | 98 | 95-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| BCEOD1OD2AverageCorrected 2002.4022.4352.4192.259 1001.7021.7891.7461.586 500.9661.0260.9960.836 250.5750.5960.5860.426 12.50.3490.3640.3570.197 6.250.2880.3050.2970.137 3.120.2090.2010.2050.045 00.1650.1550.160 | ||||||
货期：3-5 working days
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