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产品描述：人巨泌乳素酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MPRL含量。人巨泌乳素是一种由垂体前叶分泌的激素，主要功能是刺激乳汁生成和分泌。其靶点背景涉及多种生物过程，包括生殖和母乳喂养。研究机制显示，人巨泌乳素通过其受体PRLR发挥作用，影响细胞增殖、分化、分泌和生存，并在多种生理和病理生理过程中扮演重要角色。试剂盒检测范围为0.4 μIU/mL-20 μIU/mL，可广泛用于科研领域中对巨泌乳素表达水平的动态监测，例如探究其在细胞分泌调控、组织生理病理状态中的生物学功能，或用于体外实验模型中药物干预、基因编辑等因素对巨泌乳素合成与释放的影响机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MPRL
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.4 μIU/mL-20 μIU/mL
灵敏度：0.5 μIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human macroprolactin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 90-98 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-103 | |||||
回收率：
| The recovery of human macroprolactin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-102 | ||||
| EDTA plasma (n=4) | 97 | 87-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μIU/mlOD1OD2AverageCorrected202.0032.0312.0171.98781.0951.1051.1001.07030.6170.6240.6210.59110.2950.3030.2990.2690.40.1450.1490.1470.11700.0290.0310.030 | ||||||
货期：3-5 working days
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