Human Obestatin ELISA Kit

产品描述：人肥胖抑制素酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液、组织匀浆样本中的Obestatin含量。Obestatin是一种由胃部合成的23个氨基酸组成的酰胺化脑肠肽，与孤儿G蛋白偶联受体GPR39结合，具有抑制食欲、减缓体重增加、抑制胃排空和小肠收缩的生物学功能。Obestatin与Ghrelin同源于Ghrelin基因，但作用相反，Obestatin可能通过GPR39受体调节胃肠动力。试剂盒检测范围为4.69 pg/mL-300 pg/mL，本试剂盒适用于体外科研场景，例如探究肥胖相关代谢疾病机制、胃肠功能调控或内分泌信号通路研究，研究者可通过标准曲线精确测定样本中Obestatin的动态浓度变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

缩写：Obestatin

种属：Homo sapiens (Human)

样本类型：serum, plasma, cell culture supernates, urine, tissue homogenates

检测范围：4.69 pg/mL-300 pg/mL

灵敏度：1.17 pg/mL

反应时间：1-5h

样本体积：50-100ul

检测波长：450 nm

研究领域：Signal Transduction

测定原理：quantitative

测定方法：Sandwich

精密度：Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.  

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Obestatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

回收率：

The recovery of human Obestatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

货期：3-5 working days