首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠乙酰辅酶A酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的acetyl-CoA含量。Acetyl - CoA即乙酰辅酶A，是生物体代谢关键物质，在糖、脂肪、蛋白质代谢中起枢纽作用。研究发现，它参与三羧酸循环、脂肪酸合成等多种代谢途径，其水平变化会影响细胞代谢平衡，也与糖尿病、癌症等疾病的发生发展可能相关。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于研究能量代谢、脂肪酸合成及细胞调控等机制，特别针对小鼠模型中的乙酰辅酶A代谢通路探索，例如在代谢性疾病、营养干预或药物作用等科研场景中，可通过多种生物样本进行定量分析，为体外实验和离体组织研究提供可靠工具。满足科研工作者对乙酰辅酶A相关分子机制的高通量研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：acetyl-CoA
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Acetyl-CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 91 | ||||
| Range % | 87-93 | |||||
| 1:40 | Average % | 103 | ||||
| Range % | 100-105 | |||||
| 1:80 | Average % | 93 | ||||
| Range % | 87-96 | |||||
| 1:160 | Average % | 98 | ||||
| Range % | 93-101 | |||||
回收率：
| The recovery of mouse Acetyl-CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 96 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 502.1922.1142.1531.996 251.6321.6471.6401.483 12.51.1241.1101.1170.960 6.250.7130.6880.7010.544 3.120.4040.4210.4130.256 1.560.2910.2780.2850.128 0.780.2200.2120.2160.059 00.1580.1560.157 | ||||||
货期：3-5 working days
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