首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人紧密连接蛋白(Ocln)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的OCLN含量。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，可广泛应用于基础科研领域，例如研究肠道炎症模型中Ocln的动态变化、评估药物或病原体对屏障功能的调控机制、探索肿瘤微环境中细胞连接蛋白的分子互作等，为体外实验提供稳定可靠的定量检测工具。试剂盒兼容多种生物样本类型，操作流程标准化，实验数据重复性良好，满足细胞生物学、病理机制及药理学研究中对Ocln蛋白表达水平的精准分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：OCLN; Occludin
缩写：OCLN
Uniprot No.：Q16625
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human OCLN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-90 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 84-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-100 | |||||
回收率：
| The recovery of human OCLN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 80-98 | ||||
| EDTA plasma (n=4) | 94 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.2162.2522.2342.0607501.9281.8061.8671.6933751.5771.4811.5291.355187.51.2741.1931.2341.060940.7130.6940.7040.530470.4270.3950.4110.23723.50.3140.3010.3080.13400.1810.1670.174 | ||||||
货期：3-5 working days
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