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品描述：人嗅觉素样蛋白2B(OLFML2B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的OLFML2B含量。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，科研人员可通过该产品研究OLFML2B在不同生理或病理条件下的表达调控，例如探究其在特定组织中的分布特征、疾病动物模型中的动态变化，或与相关信号通路的潜在关联性，为神经生物学、发育调控及疾病机制研究提供重要实验依据，助力新型生物标志物的功能探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：OLFML2B ELISA Kit; Olfactomedin-like protein 2B ELISA Kit; Photomedin-2 ELISA Kit
缩写：OLFML2B
Uniprot No.：Q68BL8
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human OLFML2B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 87-98 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 98-104 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 89-97 | ||||||
回收率：
| The recovery of human OLFML2B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 91-99 | |||||
| EDTA plasma (n=4) | 92 | 90-95 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected40002.0252.1012.0631.92820001.3881.4251.4071.27210000.9780.9220.9500.8155000.6520.6350.6440.5092500.4660.4470.4570.3221250.3680.3750.3720.23762.50.2740.2810.2780.14300.1360.1330.135 | |||||||
货期：3-5 working days
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