首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠氧化低密度脂蛋白(OxLDL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的OxLDL含量。OxLDL即氧化低密度脂蛋白，是动脉粥样硬化发生发展的关键因素。其背景在于体内LDL被氧化修饰形成OxLDL。研究机制显示，OxLDL可被巨噬细胞摄取，形成泡沫细胞，促进脂质条纹和斑块形成，还能引发炎症反应，加速动脉粥样硬化进程。试剂盒检测范围为0.45 nmol/mL-30 nmol/mL，适用于体外实验中对小鼠模型心血管疾病机制、脂质代谢异常或氧化应激相关研究的定量分析，尤其适用于动脉粥样硬化动物模型中的动态监测及药物干预效果评估本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：OxLDL
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.45 nmol/mL-30 nmol/mL
灵敏度：0.11 nmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:400 | Average % | 85 | ||||
| Range % | 81-87 | |||||
| 1:800 | Average % | 89 | ||||
| Range % | 83-96 | |||||
回收率：
| The recovery of mouse OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-96 | ||||
| EDTA plasma (n=4) | 95 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| nmol/mlOD1OD2AverageCorrected302.6482.7722.7102.571152.1392.1302.1351.9967.51.5271.6391.5831.4443.750.8850.8660.8760.7371.860.5410.5320.5370.3980.940.3780.4050.3920.2530.450.2430.2420.2430.10400.1360.1420.139 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse OxLDL antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled OxLDL antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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