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产品描述:猪氧化低密度脂蛋白(OxLDL)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆样本中的OxLDL含量。OxLDL(氧化低密度脂蛋白)是一种被氧化修饰的低密度脂蛋白。它与动脉粥样硬化等疾病密切相关。,OxLDL可被巨噬细胞摄取形成泡沫细胞,促进脂质斑块形成,还能引发炎症反应、影响血管内皮功能,进而参与疾病的发生发展。试剂盒检测范围为0.39 nmol/mL-100 nmol/mL,可为心血管疾病机制研究及氧化应激相关模型评估提供关键数据;适用于基础科研中探究猪模型动脉粥样硬化病理进程、评估抗氧化干预效果或代谢性疾病相关研究,为心血管及代谢领域研究者提供稳定可靠的氧化应激生物标志物检测方案。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
缩写:OxLDL
种属:Sus scrofa (Pig)
样本类型:serum, plasma
检测范围:0.39 nmol/mL-100 nmol/mL
灵敏度:0.39 nmol/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Others
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of pig OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:200 | Average % | 97 | |||||
Range % | 92-105 | ||||||
1:400 | Average % | 95 | |||||
Range % | 83-100 | ||||||
1:800 | Average % | 95 | |||||
Range % | 89-100 | ||||||
1:1600 | Average % | 89 | |||||
Range % | 85-99 |
回收率:
The recovery of pig OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 95 | 90-100 | |||||
EDTA plasma (n=4) | 94 | 87-98 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
nmol/mlOD1OD2Average1000.3490.3420.346250.6270.6380.6336.250.9250.9140.921.561.1361.1531.1450.391.5941.6251.6101.8991.8641.882 |
货期:3-5 working days
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