首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Ⅰ型前胶原N端前肽(PⅠNP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PⅠNP含量。PⅠNP是一种重要靶点。其与骨代谢密切相关，可反映成骨细胞活性。研究机制上，通过检测血液中其含量变化，能评估骨形成状态，在骨质疏松等骨骼疾病的诊断、病情监测及治疗效果评估等研究中发挥着关键作用。试剂盒检测范围为4.7 pg/mL-300 pg/mL，PⅠNP可作为骨形成和纤维化进程的关键生物标志物，在骨代谢研究、纤维化疾病模型评估及药物干预效果分析中具有重要科研价值。适用于研究骨发育异常、骨质疏松模型、肝/肺纤维化机制探索等科研领域，为相关疾病模型的生物标志物分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：procollagen 1 N-terminal peptide,P1NP,_x000D_ procollagen I N-terminal peptide,PINP
缩写：PⅠNP
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：4.7 pg/mL-300 pg/mL
灵敏度：1.17 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PINP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:400 | Average % | 91 | ||||
| Range % | 85-96 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 88-102 | |||||
回收率：
| The recovery of mouse PINP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-99 | ||||
| EDTA plasma (n=4) | 89 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 3002.4132.3132.3632.261 1501.5981.4981.5481.446 750.9400.8900.9150.813 37.50.4890.4790.4840.382 18.750.3160.3060.3110.209 9.40.2070.2050.2060.104 4.70.1450.1420.1440.042 00.1030.1010.102 | ||||||
货期：3-5 working days
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