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产品描述：犬前列腺素E2(PGE2)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PG-E2含量。PG-E2即前列腺素E2，在人体生理与病理过程中广泛参与。它参与炎症反应、免疫调节等，还与肿瘤微环境形成相关。研究机制上，主要通过与细胞表面受体结合，激活信号通路，影响细胞增殖、凋亡等过程，是疾病治疗研究的潜在靶点。试剂盒检测范围为10 pg/mL-2000 pg/mL，该产品特别适用于研究犬类炎症性疾病（如关节炎、胃肠炎）、免疫应答机制、药物抗炎效果评估，以及生殖系统或肿瘤相关研究中的PGE2动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PG-E2
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：10 pg/mL-2000 pg/mL
灵敏度：5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 90-104 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-105 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-101 | |||||
回收率：
| The recovery of canine PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 85-96 | ||||
| EDTA plasma (n=4) | 96 | 89-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average20000.1870.1740.1815000.3200.3150.3181600.5170.5470.532400.8140.8480.831101.3161.3741.34501.9581.9331.946 | ||||||
货期：3-5 working days
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