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产品描述：小鼠聚合免疫球蛋白受体(PIGR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PIGR含量。PIGR即多聚免疫球蛋白受体，在黏膜免疫中发挥关键作用。它能介导多聚免疫球蛋白跨上皮转运，分泌至黏膜表面，防御病原体入侵。目前研究聚焦其转运机制、与疾病关联及作为治疗靶点潜力，有望为黏膜免疫相关疾病诊疗提供新思路。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，科研实验中定量分析不同生理或病理条件下PIGR的表达水平变化，例如黏膜免疫应答研究、肠道/呼吸道感染模型构建、免疫调节药物筛选及黏膜相关疾病机制探索等场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pigr; Polymeric immunoglobulin receptor; PIgR; Poly-Ig receptor
缩写：PIGR
Uniprot No.：O70570
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PIGR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 90 | |||||
| Range % | 85-94 | ||||||
| 1:200 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
| 1:400 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:800 | Average % | 102 | |||||
| Range % | 98-109 | ||||||
回收率：
| The recovery of mouse PIGR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 91-100 | |||||
| EDTA plasma (n=4) | 91 | 85-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20001.9251.8671.8961.75710001.3661.2821.3241.1855000.8720.8490.8610.7222500.5510.5460.5490.4101250.4150.4280.4220.28362.50.2840.2910.2880.14931.250.2310.2240.2280.08900.1420.1350.139 | |||||||
货期：3-5 working days
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