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产品描述：人丙酮酸激酶M2型同工酶(M2-PK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PKM2含量。PKM2即丙酮酸激酶M2型，是糖酵解途径的关键限速酶之一。它在肿瘤细胞中高表达，可通过调控糖代谢、参与基因转录调控等机制，为肿瘤细胞增殖提供能量和生物合成前体，因此成为癌症治疗领域的重要研究靶点。试剂盒检测范围为0.9 U/mL-60 U/mL，适用于细胞能量代谢调控相关研究；是研究肿瘤代谢重编程、药物作用机制及疾病模型构建的重要靶标；可精准检测多种生物样本中的M2 - PK浓度，支持体外培养细胞模型、动物模型组织样本以及体液样本的定量分析，为探索肿瘤微环境代谢特征、评估靶向代谢通路化合物的干预效果提供可靠工具；适用于高通量筛选实验或基础科研中能量代谢动态监测，满足肿瘤生物学、药理学及分子机制研究的需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CTHBP ELISA Kit; Cytosolic thyroid hormone-binding protein ELISA Kit; KPYM_HUMAN ELISA Kit; OIP-3 ELISA Kit; Opa-interacting protein 3 ELISA Kit; p58 ELISA Kit; pkm ELISA Kit; PKM1 ELISA Kit; PKM2 ELISA Kit; Pyruvate kinase 2/3 ELISA Kit; Pyruvate kinase muscle isozyme ELISA Kit; Pyruvate kinase PKM ELISA Kit; THBP1 ELISA Kit; Thyroid hormone-binding protein 1 ELISA Kit; Tumor M2-PK ELISA Kit
缩写：PKM2
Uniprot No.：P14618
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.9 U/mL-60 U/mL
灵敏度：0.225 U/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human M2-PK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-95 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 80-93 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 85-98 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human M2-PK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 93-106 | ||||
| EDTA plasma (n=4) | 90 | 84-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| U/mlOD1OD2AverageCorrected602.3112.3032.3072.115302.0351.9461.9911.799151.6841.6121.6481.4567.51.3231.2971.3101.1183.750.9570.9260.9420.7501.80.6480.5970.6230.4310.90.3120.3000.3060.11400.1960.1880.192 | ||||||
货期：3-5 working days
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