| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNIP1 |
| Uniprot No | Q15025 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 94-530aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSNVTASPTAPACPSDKPAPVQKPPSSGTS SEFEVVTPEEQNSPESSSHANAMALGPLPREDGNLMLHLQRLETTLSVCA EEPDHGQLFTHLGRMALEFNRLASKVHKNEQRTSILQTLCEQLRKENEAL KAKLDKGLEQRDQAAERLREENLELKKLLMSNGNKEGASGRPGSPKMEGT GKKAVAGQQQASVTAGKVPEVVALGAAEKKVKMLEQQRSELLEVNKQWDQ HFRSMKQQYEQKITELRQKLADLQKQVTDLEAEREQKQRDFDRKLLLAKS KIEMEETDKEQLTAEAKELRQKVKYLQDQLSPLTRQREYQEKEIQRLNKA LEEALSIQTPPSSPPTAFGSPEGAGALLRKQELVTQNELLKQQVKIFEED FQRERSDRERMNEEKEELKKQVEKLQAQVTLSNAQLKAFKDEEKAREALR QQKRKAKA |
| 预测分子量 | 52 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TNIP1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"TNIP1 inhibits selective autophagy via interaction with LC3 and Atg8 family proteins"*
**作者**: Li H, et al.
**摘要**: 本研究在大肠杆菌中表达并纯化重组TNIP1蛋白，发现其通过LC3结合结构域直接与自噬相关蛋白LC3相互作用，抑制细胞自噬通量，揭示了TNIP1在炎症调控中的新机制。
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2. **文献名称**: *"Structural basis for the interaction of TNIP1 with A20 in NF-κB signaling modulation"*
**作者**: Wagner SA, et al.
**摘要**: 通过昆虫细胞系统表达人源重组TNIP1蛋白，结合X射线晶体学解析其与A20的复合物结构，阐明TNIP1通过锌指结构域增强A20对NF-κB通路的负调控作用，为自身免疫疾病治疗提供靶点。
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3. **文献名称**: *"Recombinant TNIP1 protein attenuates TLR4-mediated inflammatory responses by destabilizing MyD88 complex"*
**作者**: Kim Y, et al.
**摘要**: 利用哺乳动物细胞表达的可溶性TNIP1重组蛋白，证明其通过竞争性结合MyD88抑制TLR4信号通路，减少促炎因子分泌，提示TNIP1蛋白在脓毒症等炎症疾病中的潜在治疗价值。
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以上文献涵盖了TNIP1重组蛋白的结构解析、相互作用机制及功能研究，涉及表达系统包括原核和真核体系，研究领域聚焦炎症与自噬调控。
TNIP1 (TNFAIP3-interacting protein 1), also known as ABIN1. is a ubiquitously expressed cellular protein involved in modulating inflammatory and immune signaling pathways. It interacts with TNFAIP3 (A20), a critical negative regulator of NF-κB activation, to suppress excessive immune responses. Structurally, TNIP1 contains ubiquitin-binding domains (UBAN) and a C-terminal AHD4 domain, enabling its role in protein-protein interactions and signal transduction. Dysregulation of TNIP1 has been linked to autoimmune diseases, including systemic lupus erythematosus, psoriasis, and rheumatoid arthritis, as well as cancer progression.
Recombinant TNIP1 protein is engineered for in vitro studies to dissect its molecular mechanisms. Produced via bacterial or mammalian expression systems, it retains functional domains necessary for binding partners like TNFAIP3. MyD88. or TRAF6. Researchers utilize it to investigate its inhibitory effects on NF-κB signaling, TLR/IL-1R pathways, or NLRP3 inflammasome activation. It also serves as a tool for mapping interaction interfaces, validating drug targets, or developing assays for therapeutic screening. Quality-controlled batches ensure consistency in biochemical assays, pull-down experiments, or structural analyses (e.g., X-ray crystallography). TNIP1 recombinant proteins contribute to understanding immune homeostasis and developing interventions for inflammation-related disorders.
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