首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠脂蛋白相关磷脂酶A2(Lp-PLA2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PLA2G7含量。PLA2G7即血小板活化因子乙酰水解酶，它能水解血小板活化因子。该靶点与炎症、心血管疾病等密切相关。研究机制方面主要聚焦于其在炎症信号传导中的作用，以及对脂质代谢的影响，探索其抑制剂有望为相关疾病治疗提供新策略。试剂盒检测范围为7.8 pg/mL-500 pg/mL，可在科研中用于评估小鼠模型心血管疾病进程、炎症相关机制研究或药物干预效果分析。为科研人员提供了一种高灵敏度的工具，可广泛用于基础医学研究、药效评价及分子机制探索等领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pla2g7 ELISA Kit; Pafah ELISA Kit; Platelet-activating factor acetylhydrolase ELISA Kit; PAF acetylhydrolase ELISA Kit; EC 3.1.1.47 ELISA Kit; 1-alkyl-2-acetylglycerophosphocholine esterase ELISA Kit; 2-acetyl-1-alkylglycerophosphocholine esterase ELISA Kit; LDL-associated phospholipase A2 ELISA Kit; LDL-PLA(2) ELISA Kit; PAF 2-acylhydrolase ELISA Kit
缩写：PLA2G7
Uniprot No.：Q60963
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Lp-PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 92 | ||||
| Range % | 87-102 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 84-107 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 83-102 | |||||
| 1:800 | Average % | 96 | ||||
| Range % | 84-105 | |||||
回收率：
| The recovery of mouse Lp-PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 86-105 | ||||
| EDTA plasma (n=4) | 96 | 88-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 5002.7152.6522.6842.565 2501.8171.9131.8651.746 1251.1131.1651.1391.020 62.50.6000.6270.6140.495 31.20.3800.3930.3870.268 15.60.2730.2920.2830.164 7.80.1920.2020.1970.078 00.1160.1220.119 | ||||||
货期：3-5 working days
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