首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠前黑色素聚集激素(PMCH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PMCH含量。PMCH即前黑素集中激素，参与调节能量代谢、睡眠觉醒等生理过程。其背景上与肥胖、睡眠障碍等疾病相关。研究机制方面，主要是通过与特定受体结合来传递信号，影响下游神经元活动，进而调控机体多项功能，是药物研发的潜在靶点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，为研究能量代谢相关疾病机制、神经内分泌调控或肥胖模型动物中的分子通路提供可靠工具；科研人员可通过对血清或血浆样本进行循环激素水平分析，或结合组织匀浆样本（如下丘脑等特定器官）探究PMCH的局部表达特征，适用于神经生物学、代谢疾病基础研究及药物干预效果评估等实验场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Pmch ELISA Kit; Mch ELISA Kit; Pro-MCH [Cleaved into: Neuropeptide-glycine-glutamic acid ELISA Kit; NGE ELISA Kit; Neuropeptide G-E); Neuropeptide-glutamic acid-isoleucine ELISA Kit; NEI ELISA Kit; Neuropeptide E-I); Melanin-concentrating hormone ELISA Kit; MCH)] ELISA Kit
缩写：PMCH
Uniprot No.：P56942
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PMCH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 86-95 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 93-106 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 93-101 | ||||||
回收率：
| The recovery of mouse PMCH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 87-95 | |||||
| EDTA plasma (n=4) | 88 | 84-93 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 4002.1882.2652.2272.098 2001.5091.6351.5721.443 1000.9700.9980.9840.855 500.5530.5630.5580.429 250.3520.3670.3600.231 12.50.2320.2440.2380.109 6.250.1820.1880.1850.056 00.1280.1290.129 | |||||||
货期：3-5 working days
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