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Pig Pancreatic triacylglycerol lipase(PNLIP) ELISA kit

  • 中文名: 猪胰腺三酰基甘油脂肪酶(PNLIP)酶联免疫试剂盒
货号: IPD-EL018262PI
Price: ¥3000
数量:
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产品详情

  • 产品描述:
    猪胰腺三酰基甘油脂肪酶(PNLIP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PNLIP含量。PNLIP即胰腺脂肪酶,是消化脂肪的关键酶。其背景在于它对膳食脂肪的消化吸收至关重要。研究机制方面,聚焦于其催化脂肪水解的过程,了解其活性调节机制,以此为靶点开发调节脂肪消化的药物,为肥胖、高脂血症等治疗提供思路。试剂盒检测范围为0.625 mU/mL-40 mU/mL,该产品适用于动物模型研究、营养学实验中胰腺功能评估、消化酶活性动态监测,以及药物或化合物对脂质代谢影响的体外分析。实验操作兼容多种生物样本类型,为探索胰腺生理机制、代谢性疾病发病机理等基础研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    PNLIPPancreatic triacylglycerol lipase ELISA Kit; PL ELISA Kit; PTL ELISA Kit; Pancreatic lipase ELISA Kit; EC 3.1.1.3 ELISA Kit
  • 缩写:
    PNLIP
  • Uniprot No.:
    P00591
  • 种属:
    Sus scrofa (Pig)
  • 样本类型:
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围:
    0.625 mU/mL-40 mU/mL
  • 灵敏度:
    0.156 mU/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Metabolism
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of pig PNLIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    94


    Range %

    86-100


    1:2

    Average %

    95


    Range %

    82-107


    1:4

    Average %

    92


    Range %

    81-103


    1:8

    Average %

    96


    Range %

    88-103


  • 回收率:

    The recovery of pig PNLIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    87-106


    EDTA plasma (n=4)

    97

    89-112


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    mU/ml

    OD1

    OD2

    Average

    Corrected


    40

    2.052

    2.137

    2.095

    2.007


    20

    1.639

    1.658

    1.649

    1.561


    10

    1.110

    1.134

    1.122

    1.034


    5

    0.753

    0.711

    0.732

    0.644


    2.5

    0.405

    0.392

    0.399

    0.311


    1.25

    0.312

    0.303

    0.308

    0.220


    0.625

    0.189

    0.181

    0.185

    0.097


    0

    0.086

    0.090

    0.088




  • 货期:
    3-5 working days

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