首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人对氧磷酶(PON)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的PON1含量。PON1即对氧磷酶 1，是一种与高密度脂蛋白相关的血清酯酶。它具有抗氧化、抗动脉粥样硬化等作用。其研究机制主要围绕它对脂质过氧化物的水解，降低氧化应激，还能影响炎症反应信号通路，在心血管疾病等研究中是重要靶点。试剂盒检测范围为31.25 mIU/mL-2000 mIU/mL，本产品适用于科研场景中PON表达水平的动态监测，例如探究疾病模型中酶活性变化、评估药物或化合物对PON表达的影响，或分析不同生理状态下机体抗氧化能力的变化规律。实验操作基于标准ELISA流程，可稳定检测多种生物样本，为研究PON在代谢综合征、动脉粥样硬化或有机磷暴露机制中的功能提供可靠工具，满足体外实验中对酶活性关联指标的高通量检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A esterase 1 ELISA Kit; A-esterase 1 ELISA Kit; Aromatic esterase 1 ELISA Kit; Arylesterase 1 ELISA Kit; Arylesterase B type ELISA Kit; ESA ELISA Kit; Esterase A ELISA Kit; K 45 ELISA Kit; K-45 ELISA Kit; MVCD5 ELISA Kit; Paraoxonase 1 ELISA Kit; Paraoxonase ELISA Kit; Paraoxonase B type ELISA Kit; Paraoxonase, plasma ELISA Kit; Paraoxonase1 ELISA Kit; PON 1 ELISA Kit; PON ELISA Kit; PON1 ELISA Kit; PON1_HUMAN ELISA Kit; Serum aryldiakylphosphatase ELISA Kit; Serum aryldialkylphosphatase 1 ELISA Kit; Serum paraoxonase/arylesterase 1 ELISA Kit
缩写：PON1
Uniprot No.：P27169
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 mIU/mL-2000 mIU/mL
灵敏度：7.81 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PON in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 88 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:400 | Average % | 103 | ||||
| Range % | 92-110 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human PON spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected20002.6552.6032.6292.52110002.2922.2482.2832.1755001.6891.6631.6761.5682501.0250.9891.0070.8991250.5970.6030.6000.49262.50.4090.3980.4040.29631.250.2180.2290.2240.11600.1090.1070.108 | ||||||
货期：3-5 working days
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