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产品描述：人过氧化物酶体增殖物激活受体α(PPAR-α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PPARA含量。PPARA即过氧化物酶体增殖物激活受体α，是核激素受体超家族成员。它参与脂肪酸氧化、能量代谢等生理过程。研究机制方面，PPARA可被配体激活，调节相关基因表达，在脂质代谢、炎症反应等方面发挥作用，是代谢性疾病等研究的重要靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，该产品适用于实验室基础研究，例如探索代谢综合征、动脉粥样硬化或非酒精性脂肪肝等疾病模型中PPAR-α的调控机制，也可用于药物开发过程中靶向PPAR-通路的化合物筛选与功能验证本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：hPPAR ELISA Kit; MGC2237 ELISA Kit; MGC2452 ELISA Kit; NR1C1 ELISA Kit; Nuclear receptor subfamily 1 group C member 1 ELISA Kit; OTTHUMP00000197740 ELISA Kit; OTTHUMP00000197741 ELISA Kit; Peroxisome proliferative activated receptor alpha ELISA Kit; Peroxisome proliferator activated receptor alpha ELISA Kit; Peroxisome proliferator-activated receptor alpha ELISA Kit; PPAR ELISA Kit; PPAR-alpha ELISA Kit; ppara ELISA Kit; PPARA_HUMAN ELISA Kit; PPARalpha ELISA Kit
缩写：PPARA
Uniprot No.：Q07869
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PPAR-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human PPAR-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 98 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.8062.8692.8382.74425002.0702.0982.0841.99012501.1371.1681.1531.0596250.5980.5780.5880.4943120.3150.3210.3180.2241560.2190.2290.2240.130780.1570.1590.1580.06400.0950.0930.094 | ||||||
货期：3-5 working days
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