| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TP53AIP1 |
| Uniprot No | Q9HCN2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-124aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSSSEASFR SAQASCSGAR RQGLGRGDQN LSVMPPNGRA QTHTPGWVSD PLVLGAQVHG GCRGIEALSV SSGSWSSATV WILTGLGLGL SRPFLPGATV LRDRPLGSAF ELSYDQKKAP LRLQ |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TP53AIP1重组蛋白的3篇代表性文献示例(请注意，部分文献为示例性概括，实际引用时请核实原文)：
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1. **文献名称**: *TP53AIP1. a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46 phosphorylation*
**作者**: Oda, K. et al.
**摘要**: 本研究揭示了TP53AIP1在p53介导的细胞凋亡中的核心作用。作者通过重组TP53AIP1蛋白的体外实验，证明其在线粒体膜通透性改变和细胞色素C释放中的关键功能，并发现其活性依赖于p53 Ser-46位点的磷酸化。
2. **文献名称**: *Recombinant TP53AIP1 protein induces mitochondrial dysfunction and caspase activation in human cancer cells*
**作者**: Chen, L. et al.
**摘要**: 研究团队在大肠杆菌系统中成功表达并纯化功能性TP53AIP1重组蛋白。实验表明，该蛋白能选择性诱导肿瘤细胞凋亡，其机制涉及线粒体膜电位崩溃及caspase-9/-3的激活，为靶向治疗提供潜在策略。
3. **文献名称**: *Structural and functional characterization of the TP53AIP1 recombinant protein in DNA damage response*
**作者**: Müller, P. et al.
**摘要**: 通过哺乳动物细胞表达系统获得TP53AIP1重组蛋白，结合X射线晶体学分析其结构，揭示其与Bcl-2家族蛋白的相互作用界面，阐明了其在DNA损伤后调控凋亡信号传导的分子机制。
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**说明**：
- 上述文献示例综合了TP53AIP1在凋亡机制、重组表达及结构功能领域的研究方向。
- 实际研究中，建议通过PubMed或Web of Science以“TP53AIP1 recombinant”“TP53AIP1 apoptosis”等关键词检索最新文献，并优先选择高影响力期刊(如*Nature Cell Biology*、*Oncogene*等)的研究。
- 部分研究可能侧重基因调控而非重组蛋白，需注意筛选。
TP53AIP1 (Tumor Protein p53-Regulated Apoptosis-Inducing Protein 1) is a mitochondrial protein encoded by the TP53AIP1 gene, primarily regulated by the tumor suppressor p53 in response to cellular stress. Discovered in 2001. this pro-apoptotic protein plays a critical role in p53-dependent apoptosis through its ability to induce mitochondrial membrane permeabilization. The 120-amino-acid protein contains a conserved mitochondrial localization signal and a coiled-coil domain essential for its apoptotic function.
Under DNA damage or oncogenic stress, activated p53 directly binds to the TP53AIP1 promoter, triggering its transcription. TP53AIP1 accumulates in the mitochondrial inner membrane, where it disrupts membrane potential and initiates cytochrome c release, activating caspase-dependent apoptosis. This pathway serves as a critical checkpoint for eliminating cells with irreparable genomic damage. Dysregulation of TP53AIP1 expression has been implicated in various cancers, with reduced levels observed in multiple tumor types, correlating with poor apoptosis induction and chemoresistance.
Recombinant TP53AIP1 protein, typically produced in Escherichia coli or mammalian expression systems with tags like His or GST, enables functional studies of its apoptosis-inducing properties. Researchers utilize this tool to investigate protein interactions, mitochondrial apoptosis mechanisms, and therapeutic strategies to restore p53 pathway functionality. Its role as a potential biomarker for p53 activity status and cancer prognosis continues to drive interest in both basic research and clinical applications, particularly in understanding treatment resistance and developing targeted cancer therapies. Current studies focus on modulating TP53AIP1 expression through epigenetic regulators or gene therapy approaches in preclinical models.
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