首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠穿孔素/成孔蛋白(PF/PFP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的PRF1含量。PRF1 即穿孔素 1，是一种由细胞毒性淋巴细胞分泌的蛋白质。它在免疫防御、杀伤病原体及肿瘤细胞等方面发挥关键作用。研究机制上，穿孔素 1 能在靶细胞膜上形成孔道，使颗粒酶等进入靶细胞，诱导其凋亡，以维持机体免疫平衡。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于科研领域对免疫细胞活性的定量分析，例如评估细胞毒性淋巴细胞的功能状态、研究感染或肿瘤微环境中的免疫应答机制、监测免疫调节药物对穿孔素表达的影响等实验场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Prf1 ELISA Kit; Pfp ELISA Kit; Perforin-1 ELISA Kit; P1 ELISA Kit; Cytolysin ELISA Kit; Lymphocyte pore-forming protein ELISA Kit
缩写：PRF1
Uniprot No.：P10820
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PF/PFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 98 | ||||
| Range % | 93-105 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 85-100 | |||||
| 1:80 | Average % | 86 | ||||
| Range % | 81-93 | |||||
| 1:160 | Average % | 93 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of mouse PF/PFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 90-99 | ||||
| EDTA plasma (n=4) | 87 | 84-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.1722.0842.1281.9722501.7861.6931.7401.5841251.3021.2671.2851.12962.50.7850.8230.8040.64831.250.5640.5720.5680.41215.60.3620.3490.3560.2007.80.2600.2620.2610.10500.1570.1540.156 | ||||||
货期：3-5 working days
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