Sheep Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E13161Sh

Price： ￥3000

48T 96T

数量：

大包装询价

产品描述：
绵羊催乳素(PRL/LTH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PRL含量。试剂盒检测范围为5 μIU/mL-2000 μIU/mL，科研人员可借助本试剂盒探究绵羊泌乳机制、繁殖周期调控、环境应激对激素分泌的影响，或通过分析乳腺等组织匀浆中的PRL表达水平，深入研究其生理功能与代谢调控网络。为动物生理学、生殖生物学及畜牧业相关研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
缩写：
PRL
Uniprot No.：
P01240
种属：
Ovis aries (Sheep)
样本类型：
serum, plasma, tissue homogenates
检测范围：
5 μIU/mL-2000 μIU/mL
灵敏度：
5 μIU/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-99
1:2	Average %	94
	Range %	84-101
1:4	Average %	93
	Range %	85-103
1:8	Average %	90
	Range %	87-101

回收率：

The recovery of sheep PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	85-104
EDTA plasma (n=4)	95	88-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














μIU/ml	OD1	OD2	Average
2000	0.154	0.149	0.152
375	0.338	0.351	0.345
62.5	0.542	0.572	0.557
17.5	0.983	0.959	0.971
5	1.515	1.529	1.522
0	2.164	2.077	2.121