首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人蛋白C(PC)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PROC含量。人蛋白C（PC）是一种由肝脏产生的维生素K依赖性血浆蛋白，具有抗凝作用。其通过活化蛋白C（APC）与蛋白S形成复合物，灭活活化FⅤ和FⅧ，增强纤溶活性，从而防止血栓形成。PC缺陷症与多种血栓性疾病相关，其研究机制涉及基因突变、蛋白表达和功能异常。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于基础科研领域，如凝血机制研究、血栓性疾病动物模型评估、抗凝药物开发过程中PC水平的体外定量分析，以及通过检测组织匀浆探究PC在器官中的表达分布。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Activation peptide ELISA Kit; Anticoagulant protein C ELISA Kit; APC ELISA Kit; Autoprothrombin IIA ELISA Kit; Blood coagulation factor XIV ELISA Kit; EC 3.4.21.69 ELISA Kit; PC ELISA Kit; proC ELISA Kit; PROC_HUMAN ELISA Kit; PROC1 ELISA Kit; Protein C (inactivator of coagulation factors Va and VIIIa) ELISA Kit; THPH3 ELISA Kit; THPH4 ELISA Kit; Vitamin K dependent protein C ELISA Kit
缩写：PROC
Uniprot No.：P04070
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 84 | ||||
| Range % | 81-89 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:400 | Average % | 99 | ||||
| Range % | 95-102 | |||||
| 1:800 | Average % | 101 | ||||
| Range % | 98-104 | |||||
回收率：
| The recovery of human PC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-100 | ||||
| EDTA plasma (n=4) | 104 | 100-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.6042.6972.6512.5531002.1962.1742.1852.087501.5041.5351.5201.422250.9130.9340.9240.82612.50.4770.4980.4880.3906.250.3470.3680.3580.2603.120.2340.2450.2400.14200.0970.0990.098 | ||||||
货期：3-5 working days
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