关键信息
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基因名
rpsF
- 应用
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别名
rpsF; b4200; JW4158; 30S ribosomal protein S6; Small ribosomal subunit protein bS6) [Cleaved into: 30S ribosomal protein S6; fully modified isoform; 30S ribosomal protein S6; non-modified isoform]
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种属
Escherichia coli
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表达系统
E. coli
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标签
N- GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P02358
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表达区间
1-131aa
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分子量
42.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
RpsF is a crucial component of the ribosomal protein family, specifically known as protein S6 in bacteria, playing a vital role in protein synthesis and ribosome assembly. Research into the recombinant form of RpsF has gained significance due to its essential function in maintaining ribosomal stability and facilitating translation processes in prokaryotes. Investigating RpsF's structure and function can provide insights into the ribosomal machinery, aiding in the development of new antibiotics targeting bacterial ribosomes, which is increasingly important in the context of rising antibiotic resistance. Additionally, understanding the interactions between RpsF and ribosomal RNA may elucidate the mechanisms of ribosome biogenesis and regulation, opening pathways for genetic engineering applications and synthetic biology. Given its importance, the recombinant expression of RpsF in model systems allows for detailed biophysical and biochemical characterization, providing a basis for potential therapeutic strategies and biotechnological innovations. Consequently, RpsF serves as a critical model for studying ribosomal function and offers promising avenues for research in microbiology and medicinal chemistry.
