| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TPPP3 |
| Uniprot No | Q9BW30 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-176aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAASTDM AGLEESFRKF AIHGDPKASG QEMNGKNWAK LCKDCKVADG KSVTGTDVDI VFSKVKGKSA RVINYEEFKK ALEELATKRF KGKSKEEAFD AICQLVAGKE PANVGVTKAK TGGAVDRLTD TSRYTGSHKE RFDESGKGKG IAGRQDILDD SGYVSAYKNA GTYDAKVKK |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TPPP3重组蛋白的参考文献及摘要概括：
1. **标题**：*TPPP3 promotes lung cancer cell migration and invasion via EMT activation*
**作者**：Zhang Y, et al. (2021)
**摘要**：该研究通过在大肠杆菌中表达并纯化重组TPPP3蛋白，发现其能显著增强肺癌细胞的迁移和侵袭能力。实验表明，TPPP3可能通过激活上皮-间质转化(EMT)通路介导这一过程，为肺癌治疗的潜在靶点提供了依据。
2. **标题**：*Structural insights into TPPP3 and its distinct microtubule-binding mechanism*
**作者**：Smith J, et al. (2019)
**摘要**：本研究利用昆虫细胞系统表达TPPP3重组蛋白，并通过X射线晶体学解析其三维结构。结果显示，TPPP3的C端结构域与其他TPPP家族成员存在显著差异，可能解释其在微管动力学调控中的独特功能。
3. **标题**：*Phosphorylation-dependent regulation of TPPP3's microtubule-polymerizing activity in neurodegenerative models*
**作者**：Lee S, et al. (2020)
**摘要**：作者通过哺乳动物细胞表达重组TPPP3蛋白，发现其磷酸化修饰(如Ser-15位点)显著影响其促进微管聚合的能力。该发现提示TPPP3的翻译后修饰可能在神经退行性疾病的病理过程中起关键作用。
4. **标题**：*Optimized mammalian expression system enhances recombinant TPPP3 production for functional studies*
**作者**：Chen L, et al. (2022)
**摘要**：研究开发了一种基于HEK293细胞的重组TPPP3高效表达系统，通过优化载体设计和纯化策略，获得了高纯度且具有生物活性的TPPP3蛋白，为后续药物筛选及相互作用研究提供了可靠工具。
(注：上述文献信息为示例性模拟，实际引用需以真实发表文献为准。)
TPPP3 (Tubulin Polymerization Promoting Protein 3) is a member of the TPPP protein family, which is characterized by its conserved TPPP domain involved in microtubule dynamics regulation. Unlike its paralogs TPPP1 and TPPP2. TPPP3 is shorter in length and lacks certain structural motifs, but retains the core tubulin-binding region critical for modulating microtubule stability and organization. It is expressed in various tissues, including the central nervous system, testis, and epithelial cells, and plays roles in cellular processes such as cilia assembly, cell division, and cytoskeletal remodeling.
Recombinant TPP3 protein is engineered using expression systems like *E. coli* or mammalian cells to produce purified, functional protein for research. It typically includes affinity tags (e.g., His-tag) for ease of purification and detection. Studies suggest TPPP3 interacts with α/β-tubulin heterodimers, promoting microtubule polymerization or stabilization under specific conditions. Its dysregulation has been linked to pathologies, including cancers (e.g., glioblastoma, lung cancer) and ciliopathies, where aberrant microtubule dynamics contribute to disease progression.
Research on recombinant TPPP3 aids in elucidating its molecular mechanisms, such as its synergy with other microtubule-associated proteins or post-translational modifications. It also serves as a tool for drug screening, particularly for targeting microtubule-related disorders. Despite its emerging significance, TPPP3's full functional spectrum and regulatory pathways remain under investigation, highlighting its potential as a therapeutic or diagnostic target in microtubule-associated diseases.
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