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产品描述：人蛋白prune同源物(PRUNE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PRUNE含量。试剂盒检测范围为47 pg/mL-3000 pg/mL，适用于科研领域中对PRUNE蛋白功能的机制研究，例如探究其在癌症转移微环境中的作用、神经退行性疾病相关信号通路调控，或结合细胞模型分析药物干预对PRUNE表达的影响本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DRES-17 ELISA Kit; DRES17 ELISA Kit; Drosophila-related expressed sequence 17 ELISA Kit; hPrune ELISA Kit; HTcD37 ELISA Kit; Protein prune homolog ELISA Kit; PRUNE ELISA Kit; Prune homolog ELISA Kit; PRUNE like protein ELISA Kit; PRUNE_HUMAN ELISA Kit; TcD37 homolog ELISA Kit
缩写：PRUNE
Uniprot No.：Q86TP1
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PRUNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 83-94 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-104 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 93-96 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human PRUNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-96 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.2832.1832.2332.11115001.6471.5671.6071.4857500.9820.9520.9670.8453750.5320.5120.5220.400187.50.3840.3740.3790.257940.2470.2370.2420.120470.1990.1890.1940.07200.1220.1210.122 | ||||||
货期：3-5 working days
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